Gram-Scale Purification of Flavonolignan Diastereoisomers from Silybum marianum (Milk Thistle) Extract in Support of Preclinical in vivo Studies for Prostate Cancer Chemoprevention

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Milk Thistle (Silybum marianum)

Milk thistle is a well known and commonly used herb, used particularly in hepatic diseases. It has very potent antioxidant, anti-inflammatory, and antifibrotic properties (1). As a single agent its therapeutic effects in liver disease appear to be modest (2,3,4,5). Herbal medicines and supplements are likely to be much more beneficial when used synergistically in combinations or formulas. Milk ...

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Large-scale isolation of flavonolignans from Silybum marianum extract affords new minor constituents and preliminary structure-activity relationships.

The gram-scale isolation of the major flavonolignan diastereoisomers from milk thistle ( Silybum marianum) extract provided an entree into the isolation of two related analogues that are present in extremely minute quantities. The isolation and structure elucidation of these two new compounds, which we have termed isosilybin C and isosilybin D due to their structural similarities to isosilybin ...

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Silybum marianum in vitro-flavonolignan production

The effect of coniferyl alcohol as a precursor of flavonolignan biosynthesis on silymarin components production in Silybum marianum suspension culture was studied. Coniferyl alcohol showed the changes in silymarin complex production. Silydianin was detected mainly in the control samples of cultivated cells. A significant increase of silydianin was observed only after 72 h of the application of ...

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Stimulatory effects of silibinin and silicristin from the milk thistle Silybum marianum on kidney cells.

The biochemical influence of flavonolignans from the milk thistle Silybum marianum has been tested on kidney cells of African green monkeys. Two nonmalignant cell lines were selected, with the focus of the work on the fibroblast-like Vero line. Proliferation rate, biosynthesis of protein and DNA, and the activity of the enzyme lactate dehydrogenase (as a measure of the cellular metabolic activi...

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Phylogenetic and chemical diversity of fungal endophytes isolated from Silybum marianum (L) Gaertn. (milk thistle)

Use of the herb milk thistle (Silybum marianum) is widespread, and its chemistry has been studied for over 50 years. However, milk thistle endophytes have not been studied previously for their fungal and chemical diversity. We examined the fungal endophytes inhabiting this medicinal herb to determine: (1) species composition and phylogenetic diversity of fungal endophytes; (2) chemical diversit...

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ژورنال

عنوان ژورنال: Planta Medica

سال: 2007

ISSN: 0032-0943,1439-0221

DOI: 10.1055/s-2007-990239